Polymorphic offers various library construction services tailored to the specific needs of our customers. Our high-throughput facility allows for efficient handling of a large number of samples

cDNA Library Construction
Polymorphic offers the construction of plasmid-based cDNA libraries from the following customer provided starting materials:

High-quality mRNA is synthesized into cDNA using oligo dT primers. Once both strands are synthesized and annealed, the double stranded cDNA is directionally cloned into an expression vector.

Shotgun Library Construction & Sequencing
Shotgun library construction and sequencing can begin with a variety of samples such as cosmid, BAC, and YAC DNA. After a customer consultation to determine the goals and needs of a specific project, Polymorphic offers the following shotgun library service(s):

Construction:

• Plasmid DNA purification and/or quality analysis of in-coming DNA
  
• Random shearing to produce a population of DNA fragments within 1-3 KB
  
• Ligation into a standard cloning vector
  
• Transformation & glycerol stock production
  
• QA/QC by DNA sequencing

  
  Sequencing:
  

• Plating, picking, and arranging colonies into high-throughput format (96 or 384 wells)
  
• Plasmid purification of clones
  
• Sequencing assay design, primer synthesis, and bioinformatics analysis
  
• High-throughput sequencing on ABI 3730xl DNA sequencers
  
• Contig alignment and assembly

Upon conclusion of the project, the customer is provided with a report including the original electropherograms, all primer sequence information, and the full consensus sequence. All plasmid DNA and primers are delivered.