Polymorphic offers various library construction services tailored to the specific needs of our customers. Our high-throughput facility allows for efficient handling of a large number of samples

Shotgun Library Construction & Sequencing
Shotgun library construction and sequencing can begin with a variety of samples such as cosmid, BAC, and YAC DNA. After a customer consultation to determine the goals and needs of a specific project, Polymorphic offers the following shotgun library service(s):

Construction:

• Plasmid DNA purification and/or quality analysis of in-coming DNA
  
• Random shearing to produce a population of DNA fragments within 1-3 KB
  
• Ligation into a standard cloning vector
  
• Transformation & glycerol stock production
  
• QA/QC by DNA sequencing

  
  Sequencing:
  

• Plating, picking, and arranging colonies into high-throughput format (96 or 384 wells)
  
• Plasmid purification of clones
  
• Sequencing assay design, primer synthesis, and bioinformatics analysis
  
• High-throughput sequencing on ABI 3730xl DNA sequencers
  
• Contig alignment and assembly

Upon conclusion of the project, the customer is provided with a report including the original electropherograms, all primer sequence information, and the full consensus sequence. All plasmid DNA and primers are delivered.

cDNA Library Construction
Polymorphic offers the construction of plasmid-based cDNA libraries from the following customer provided starting materials:

High-quality mRNA is synthesized into cDNA using oligo dT primers. Once both strands are synthesized and annealed, the double stranded cDNA is directionally cloned into an expression vector.