Polymorphic DNA Technologies delivers quality service at each step of the SNP discovery and SNP scoring process.

Use of Sanger Sequencing in SNP Scoring
The use of DNA sequencing itself substantially contributes to the quality of our service.

The method we use to design a SNP genotyping assay always generates an amplicon that can be sequenced. There is never a question about the "assay compatibility" of any polymorphic locus. We can design and run assays for any SNP.

DNA sequencing is the accepted "Gold Standard" in DNA analysis. Most SNPs are discovered by DNA sequencing, which is considered an accurate, proven and reliable methodology.

Since the surrounding DNA sequence context is provided, our customers can be certain that the proper base has been evaluated.

Use of Sanger Sequencing in SNP Discovery
As mentioned above, DNA sequencing is the accepted "Gold Standard" in DNA analysis. Most SNPs are discovered by DNA sequencing, which is considered an accurate, proven and reliable methodology. To ensure accuracy and sensitivity, only data from the high resolution, high quality region of the DNA sequencing run are used in our discovery service.

Independent Quality Checks
To confirm the quality of a genotyping result, a sequence alignment is performed. Additionally, the sequence data must pass a threshold signal intensity and regional PHRED score to be considered a quality result. The nucleotide of interest and the surrounding bases must pass these quality checks.

Method Confirmation
Polymorphic routinely performs in-house studies analyzing SNPs among numerous family members across three generations. These genotyping results consistently show a Mendelian pattern of inheritance among the family members, confirming the accuracy and validity of our methods.

Automated Sample Handling
Polymorphic's automated systems ensure reliable sample handling throughout the SNP discovery and genotyping process. Human error is minimized by the use of robotics. Sample plates can only be loaded onto our DNA sequencing instrument in their correct orientation, preventing errors. Our liquid handling instruments perform plate to plate transfers, preventing inappropriate sample delivery. Our tests have shown sample carryover of less than 1 part per million. Samples are processed in a clean room environment.

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